Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Upregulated Talin1 synergistically boosts β-estradiol-induced proliferation and pro-angiogenesis of eutopic and ectopic endometrial stromal cells in adenomyosis

doi: 10.1186/s12958-021-00756-7

Figure Lengend Snippet: β-E 2 induced adenomyotic endometrial stromal cell proliferation in vitro. a . Cell proliferation rates of adenomyotic and normal endometrial stromal cells with or without β- E 2 . b . Numbers of formative colonies of adenomyotic and normal endometrial stromal cells with or without β- E 2 . c . and d . CCK-8 assay and colony formation assay were performed to evaluate the effects of β- E 2 on the proliferation of ADS eutopic and ectopic endometrial stromal cells. e . and f . Western blot and gray value analysis of molecular markers for cell division and proliferation including PCNA and Ki67 in ADS endometrial stromal cells treated with E 2 (10nM) or Fulvestrant (a selective estrogen receptor antagonist, 10 nM) or both or neither of them. GAPDH was used as a loading control. Data were presented as mean ± SD. NS no significance, * P < 0.05, ** P < 0.01. ADS_Eu_ESC, adenomyotic eutopic endometrial stromal cells; ADS_Ec_ESC, adenomyotic ectopic endometrial stromal cells

Article Snippet: Subsequently, cells were incubated in fresh medium (as control), β-E 2 (10nM, Sigma, USA), Fulvestrant (10 nM, ICI 182780, a selective ER antagonist, MedChem Express, USA) or β-E 2 plus Fulvestrant for 24 h.

Techniques: In Vitro, CCK-8 Assay, Colony Assay, Western Blot, Control

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Upregulated Talin1 synergistically boosts β-estradiol-induced proliferation and pro-angiogenesis of eutopic and ectopic endometrial stromal cells in adenomyosis

doi: 10.1186/s12958-021-00756-7

Figure Lengend Snippet: β-E 2 induced adenomyotic endometrial stromal cell angiogenesis in vitro. a . b . Assessment of pro-angiogenetic ability of adenomyotic and normal endometrial stromal cells with or without β- E 2 . c . d . Tube and network formation assay of HUVEC cells treated with conditioned media (the whole cell filtrate from ADS_Eu_ESC and ADS_Ec_ESC added with either E 2 (10nM) or Fulvestrant (10 nM) or both. Images were captured when HUVEC were cultured with the conditioned media on Matrigel for 6 h. Branch points of capillaries were counted and analyzed. Scale bar = 100 μm. e . f . Western blot detection of angiogenesis-related proteins including VEGFB and ANGPTL4 in ADS_Eu_ESC and ADS_Ec_ESC cells after being treated with E 2 (10nM) or Fulvestrant (10 nM). GAPDH was used as a loading control. Data were presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001. HUVEC, human umbilical vein endothelial cells

Article Snippet: Subsequently, cells were incubated in fresh medium (as control), β-E 2 (10nM, Sigma, USA), Fulvestrant (10 nM, ICI 182780, a selective ER antagonist, MedChem Express, USA) or β-E 2 plus Fulvestrant for 24 h.

Techniques: In Vitro, Tube Formation Assay, Cell Culture, Western Blot, Control